Image and file formats

ImageJ expects the image files to be 32-bit 4dfp stacks (typically with the .4dfp.img extension). It also expects to find corresponding header files (with the .ifh extension). FIV can handle images that have been transformed into 111, 222, or 333 space. The coordinates displayed in the toolbar panel and popup windows will adjust automatically to the appropriate voxel size as long as the header files are available. If the header files are missing or corrupt, FIV will attempt to determine an appropriate voxel size from the file name and file size, but there’s no guarantee that the resulting coordinates and image display will be correct.